Based on my non scientific observations, and the reports of others, I believe that the first hand cycled round (either sling shot'd or from slide stop) may hit to a different point of impact than a first cartridge that has been cycled by the firing process.
I hope to try to test this with different pistols as time allows, but I am throwing this out there in case others can try to observe or test this in their own group shooting. If it is a "thing" you may be able to incorporate this into your planning, in terms of the order you address targets or the condition you leave your pistol in.
Please post pictures or observations.